abstract：:Ribosome recycling usually occurs after canonical termination triggered by a stop codon. Additionally, ribosomes that are stalled by aberrant mRNAs need to be recognized and subsequently recycled. In eukaryotes and archaea, the factors involved in canonical termination and ribosome rescue are structurally and function...

journal_title：Current opinion in structural biology

authors： Franckenberg S,Becker T,Beckmann R

更新日期：2012-12-01 00:00:00

abstract：:Since its debut in the mid 1970s, electron crystallography has been a valuable alternative in the structure determination of biological macromolecules. Its reliance on single-layered or double-layered two-dimensionally ordered arrays and the ability to obtain structural information from small and disordered crystals m...

journal_title：Current opinion in structural biology

authors： Pope CR,Unger VM

更新日期：2012-08-01 00:00:00

abstract：:The bacterial cholesterol dependent cytolysins (CDCs) and membrane attack complex/perforin-like proteins (MACPF) represent two major branches of a large, exceptionally diverged superfamily. Most characterized CDC/MACPF proteins form large pores that function in immunity, venoms, and pathogenesis. Extensive structural,...

journal_title：Current opinion in structural biology

authors： Dunstone MA,Tweten RK

更新日期：2012-06-01 00:00:00

abstract：:Protein-RNA interactions play essential roles in gene regulation and RNA metabolism. While high-resolution structures have revealed principles of RNA recognition by individual RNA binding domains (RBDs), the presence of multiple RBDs in many eukaryotic proteins suggests additional modes of RNA recognition by combinati...

journal_title：Current opinion in structural biology

authors： Mackereth CD,Sattler M

更新日期：2012-06-01 00:00:00

abstract：:As the fundamental packing units of DNA in eukaryotes, nucleosomes play a central role in governing DNA accessibility in a variety of cellular processes. Our understanding of the mechanisms underlying this complex regulation has been aided by unique structural and dynamic perspectives offered by single molecule techni...

journal_title：Current opinion in structural biology

authors： Killian JL,Li M,Sheinin MY,Wang MD

更新日期：2012-02-01 00:00:00

abstract：:Protein aggregation constitutes a constant threat for the living cell as is demonstrated by the several pathologies in which the mechanisms to prevent it fail. It is therefore a question of increasing importance to understand in detail the defence strategies. Here we discuss how molecular interactions can represent a ...

journal_title：Current opinion in structural biology

authors： Pastore A,Temussi PA

更新日期：2012-02-01 00:00:00

abstract：:This review focuses on recent structural insights into regulation and nucleic acid binding of Superfamily 2 (SF2)-type helicases as they relate to chromatin remodelers. We review structural features of the Chd1 chromatin remodeler regarding regulation of the ATPase motor, and discuss related strategies observed for ot...

journal_title：Current opinion in structural biology

authors： Hauk G,Bowman GD

更新日期：2011-12-01 00:00:00

abstract：:Eukaryotic transcriptional coactivators are multi-subunit complexes that both modify chromatin and recognize histone modifications. Until recently, structural information on these large complexes has been limited to isolated enzymatic domains or chromatin-binding motifs. This review summarizes recent structural studie...

journal_title：Current opinion in structural biology

authors： Samara NL,Wolberger C

更新日期：2011-12-01 00:00:00

abstract：:Histones are highly positively charged proteins that wrap our genome. Their surface properties also make them prone to nonspecific interactions and aggregation. A class of proteins known as histone chaperones is dedicated to safeguard histones by aiding their proper incorporation into nucleosomes. Histone chaperones f...

journal_title：Current opinion in structural biology

authors： Hondele M,Ladurner AG

更新日期：2011-12-01 00:00:00

abstract：:Membranes are sites of intense signaling activity within the cell, serving as dynamic scaffolds for the recruitment of signaling molecules and their substrates. The specific and reversible localization of these signaling molecules to membranes is critical for the appropriate activation of downstream signaling pathways...

journal_title：Current opinion in structural biology

authors： Leonard TA,Hurley JH

更新日期：2011-12-01 00:00:00

abstract：:Kinetochores are large macromolecular assemblies that link chromosomes to spindle microtubules (MTs) during mitosis. Here we review recent advances in the study of core MT-binding kinetochore complexes using electron microcopy methods in vitro and nanometer-accuracy fluorescence microscopy in vivo. We synthesize these...

journal_title：Current opinion in structural biology

authors： Alushin G,Nogales E

更新日期：2011-10-01 00:00:00

abstract：:Over the past two decades, mass spectrometry (MS) has emerged as a bone fide approach for structural biology. MS can inform on all levels of protein organization, and enables quantitative assessments of their intrinsic dynamics. The key advantages of MS are that it is a sensitive, high-resolution separation technique ...

journal_title：Current opinion in structural biology

authors： Benesch JL,Ruotolo BT

更新日期：2011-10-01 00:00:00

abstract：:A network of dynamic protein interactions with their protein partners, substrates, and ligands is known to be crucial for biological function. Revealing molecular and structural-based mechanisms at atomic resolution and in real-time is fundamental for achieving a basic understanding of cellular processes. These techni...

journal_title：Current opinion in structural biology

authors： Grossman M,Sela-Passwell N,Sagi I

更新日期：2011-10-01 00:00:00

abstract：:Directed evolution is widely used to improve enzymes, particularly for industrial biocatalytic processes. Molecular biology advances present many new strategies for directed evolution. Commonly used techniques have led to many successful examples of enzyme improvement, yet there is still a need to improve both the eff...

journal_title：Current opinion in structural biology

authors： Dalby PA

更新日期：2011-08-01 00:00:00

abstract：:Condensation of DNA in the nucleosome takes advantage of its double-helical architecture. The DNA deforms at sites where the base pairs face the histone octamer. The largest so-called kink-and-slide deformations occur in the vicinity of arginines that penetrate the minor groove. Nucleosome structures formed from the 6...

journal_title：Current opinion in structural biology

authors： Olson WK,Zhurkin VB

更新日期：2011-06-01 00:00:00

abstract：:Improved rational drug design methods are needed to lower the cost and increase the success rate of drug discovery and development. Alchemical binding free energy calculations, one potential tool for rational design, have progressed rapidly over the past decade, but still fall short of providing robust tools for pharm...

journal_title：Current opinion in structural biology

authors： Chodera JD,Mobley DL,Shirts MR,Dixon RW,Branson K,Pande VS

更新日期：2011-04-01 00:00:00

abstract：:High-throughput interaction discovery initiatives have revealed the existence of hundreds of multiprotein complexes whose functions are regulated through thousands of protein-protein interactions (PPIs). However, the structural details of these interactions, often necessary to understand their function, are only avail...

journal_title：Current opinion in structural biology

authors： Stein A,Mosca R,Aloy P

更新日期：2011-04-01 00:00:00

abstract：:Computational protein design - the search for amino acids that adopt defined structures and functions - has achieved significant successes in recent years. Design is often approached from the perspective of targeting the design objective one structure-sequence pair at a time. Here we summarize studies that take an alt...

journal_title：Current opinion in structural biology

authors： Friedland GD,Kortemme T

更新日期：2010-06-01 00:00:00

abstract：:Changing macromolecular conformations and complexes are critical features of cellular networks, typified by DNA damage response pathways that are essential to life. These fluctuations enhance the specificity of macromolecular recognition and catalysis, and enable an integrated functioning of pathway components, ensuri...

journal_title：Current opinion in structural biology

authors： Perry JJ,Cotner-Gohara E,Ellenberger T,Tainer JA

更新日期：2010-06-01 00:00:00

abstract：:Recent collection of high-resolution crystal structures of the 70S ribosome with mRNA and tRNA substrates enhances our knowledge of protein synthesis principles. A novel network of interactions between the ribosome in the elongation state and mRNA downstream from the A codon suggests that mRNA is stabilized and aligne...

journal_title：Current opinion in structural biology

authors： Demeshkina N,Jenner L,Yusupova G,Yusupov M

更新日期：2010-06-01 00:00:00

abstract：:Pore-forming proteins (PFPs), involved in host-pathogen interactions, are produced as soluble, generally monomeric, proteins. To convert from the soluble to the transmembrane form, PFPs assemble, in the vicinity of the target membrane, into ring-like structures, which expose sufficient hydrophobicity to drive spontane...

journal_title：Current opinion in structural biology

authors： Iacovache I,Bischofberger M,van der Goot FG

更新日期：2010-04-01 00:00:00

abstract：:RNA-dependent RNA polymerases (RdRps) of the segmented double-stranded (ds) RNA viruses of the Reoviridae family exhibit distinguishing structural elements, enabling the enzymes to function within the confines of a proteinaceous core particle. These globular, cage-like polymerases are traversed by four well-defined tu...

journal_title：Current opinion in structural biology

authors： McDonald SM,Tao YJ,Patton JT

更新日期：2009-12-01 00:00:00

abstract：:RNA-dependent RNA polymerases (RdRPs) play central roles in both transcription and viral genome replication. In picornaviruses, these functions are catalyzed by the virally encoded RdRP, termed 3D. Polymerase 3D also catalyzes the covalent linkage of UMP to a tyrosine on the small protein VPg. Uridylylated VPg then se...

journal_title：Current opinion in structural biology

authors： Ferrer-Orta C,Agudo R,Domingo E,Verdaguer N

更新日期：2009-12-01 00:00:00

abstract：:Mass spectrometry is becoming a more and more powerful tool for investigating protein complexes. Recent developments, based on different ionization techniques, electrospray, desorption/ionization and others are contributing to the usefulness of MS to describe the organization and structure of large non-covalent assemb...

journal_title：Current opinion in structural biology

authors： Bich C,Zenobi R

更新日期：2009-10-01 00:00:00

abstract：:The wide occurrence of sialic acids (Sia) in various chemical forms linked as monomers or polymers in an outstanding position in a multitude of complex carbohydrates of animals and microorganisms renders them as most versatile function modulators in cell biology and pathology. A survey is presented of recent advances ...

journal_title：Current opinion in structural biology

authors： Schauer R

更新日期：2009-10-01 00:00:00

abstract：:Membrane protein structural biology is enjoying a steady acceleration in the rate of success. Nevertheless, numerous membrane protein targets are resistant to the traditional approach of directly crystallizing detergent solubilized and purified protein and the 'niche market' of lipidic phase crystallization is emergin...

journal_title：Current opinion in structural biology

authors： Johansson LC,Wöhri AB,Katona G,Engström S,Neutze R

更新日期：2009-08-01 00:00:00

abstract：:The X-ray structures of two prokaryotic pentameric ligand-gated ion channels have recently provided detailed insight into this important family of neurotransmitter receptors. These prokaryotic homologs share the overall architecture of their eukaryotic counterparts with conservation in functionally important residues....

journal_title：Current opinion in structural biology

authors： Hilf RJ,Dutzler R

更新日期：2009-08-01 00:00:00

abstract：:The modulation of enzyme function is a key regulatory feature of biological systems. The ability to engineer synthetic enzymes that can be controlled by any arbitrary signal would enable a wide array of sensing applications and therapeutics and provide us with powerful tools for the basic study of biology. Here severa...

journal_title：Current opinion in structural biology

authors： Ostermeier M

更新日期：2009-08-01 00:00:00

abstract：:Photolyases repair cytotoxic and mutagenic UV-induced photolesions in DNA by using an amazing light-dependent repair mechanism. It involves light absorption, electron transfer from an excited reduced and deprotonated FADH(-) to the flipped-out photolesion, followed by the fragmentation of the photolesions. Cryptochrom...

journal_title：Current opinion in structural biology

authors： Müller M,Carell T

更新日期：2009-06-01 00:00:00

abstract：:You want to know how proteins do it? Take a walk in protein fold space. More often than not you will get a clue if not the answer. If you know what you are looking for and how to find it. In fact, there is more information than we can presently handle. Charting fold space and chasing its creatures has occupied us for ...

journal_title：Current opinion in structural biology

authors： Sippl MJ

更新日期：2009-06-01 00:00:00

abstract：:Intron splicing is a fundamental biological process whereby noncoding sequences are removed from precursor RNAs. Recent work has provided new insights into the structural features and reaction mechanisms of two introns that catalyze their own splicing from precursor RNA: the group I and II introns. In addition, there ...

journal_title：Current opinion in structural biology

authors： Toor N,Keating KS,Pyle AM

更新日期：2009-06-01 00:00:00

abstract：:Molecular dynamics simulations allow for atomic-level characterization of biomolecular processes such as the conformational transitions associated with protein function. The computational demands of such simulations, however, have historically prevented them from reaching the microsecond and greater timescales on whic...

journal_title：Current opinion in structural biology

authors： Klepeis JL,Lindorff-Larsen K,Dror RO,Shaw DE

更新日期：2009-04-01 00:00:00

abstract：:PUF proteins are RNA-binding proteins named for founding members PUMILIO and fem-3 binding factor (FBF). Together these proteins represent the range of known RNA recognition properties. PUMILIO is a prototypical PUF protein whose RNA sequence specificity is simple, elegant, and predictable. FBF displays differences in...

journal_title：Current opinion in structural biology

authors： Lu G,Dolgner SJ,Hall TM

更新日期：2009-02-01 00:00:00

abstract：:The RNA exosome participates in the degradation and processing of a wide range of RNA molecules. Recent advances in understanding how the exosome is organized and functions largely stem from structural studies. Crystal structures of archaeal exosomes bound to RNA and of the corresponding nine-subunit human exosome cor...

journal_title：Current opinion in structural biology

authors： Lorentzen E,Basquin J,Conti E

更新日期：2008-12-01 00:00:00

abstract：:Coiled coils have a rich history in the field of protein design and engineering. Novel structures, such as the first seven-helix coiled coil, continue to provide surprises and insights. Large-scale datasets quantifying the influence of systematic mutations on coiled-coil stability are a valuable new asset to the area....

journal_title：Current opinion in structural biology

authors： Grigoryan G,Keating AE

更新日期：2008-08-01 00:00:00

abstract：:Molecular dynamics simulations of membrane proteins are making rapid progress, because of new high-resolution structures, advances in computer hardware and atomistic simulation algorithms, and the recent introduction of coarse-grained models for membranes and proteins. In addition to several large ion channel simulati...

journal_title：Current opinion in structural biology

authors： Lindahl E,Sansom MS

更新日期：2008-08-01 00:00:00

abstract：:The observed distribution of protein structures can give us important clues about the underlying evolutionary process, imposing important constraints on possible models. The availability of results from an increasing number of genome projects has made the development of these models an active area of research. Models ...

journal_title：Current opinion in structural biology

authors： Goldstein RA

更新日期：2008-04-01 00:00:00

abstract：:Molecular dynamics simulation studies of protein-nucleic acid complexes are more complicated than studies of either component alone-the force field has to be properly balanced, the systems tend to become very large, and a careful treatment of solvent and of electrostatic interactions is necessary. Recent investigation...

journal_title：Current opinion in structural biology

authors： Mackerell AD Jr,Nilsson L

更新日期：2008-04-01 00:00:00

abstract：:Polyketide synthases (PKSs) form a large family of multifunctional proteins involved in the biosynthesis of diverse classes of natural products. Architecturally at least three different types of PKSs have been discovered in the microbial world and recent years have revealed tremendous versatility of PKSs, both in term...

journal_title：Current opinion in structural biology

authors： Gokhale RS,Sankaranarayanan R,Mohanty D

更新日期：2007-12-01 00:00:00

abstract：:Almost two decades after their identification, the components of the mitotic checkpoint are finally revealing their structural secrets. The activation of Mad2, a central piece of the checkpoint protein machinery, is linked to the rare ability of this protein to adopt two distinct topologies. Current models of checkpoi...

journal_title：Current opinion in structural biology

authors： Mapelli M,Musacchio A

更新日期：2007-12-01 00:00:00